Detection of superoxide anion release by endothelial cells using reduced cytochrome c UV-visible absorption, ESR spin trapping,hydroethidine fluorescence and lucigenin-enhanced chemiluminescence techniques
نویسندگان
چکیده
منابع مشابه
Nitric oxide and superoxide in biological systems
Oxygen-derived free radicals play a major role in the physiology and pathology of the vessel wall [1]. To assess superoxide anion production (O2) from endothelium, four techniques are mainly used: reduced cytochrome c UV-visible absorption [2,3], ESR spin trapping [4,5], hydroethydine fluorescence [6] and lucigenin-enhanced chemiluminescence [7,8]. The simplest technique which has been used for...
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Superoxide Detection To the Editor: The recent review by Tarpey and Fridovich published in Circulation Research1 mostly contains interesting and sound material; however, I would like to comment on the section entitled, “Superoxide Detection, Chemiluminescence Reactions.” Basing mainly on their own data,2 authors maintain that lucigenin-amplified chemiluminescence (LucCL) overestimates the rate ...
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Lucigenin (LC2+) is frequently used as a superoxide probe. To detect superoxide, lucigenin must be reduced to the lucigenin cation radical (LC.+). We show, using the phosphorylated spin trap 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), that lucigenin stimulates NADPH-dependent superoxide production by endothelial nitric oxide synthase (eNOS). The formation of the DEPMPO-superoxid...
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The superoxide radical anion (SO) is a critical biomarker for monitoring cellular stress responses. Electrochemical SO biosensors are frequently constructed through the covalent immobilization of cytochrome c (Cyt c) onto self-assembled monolayers (SAMs); however, a detailed comparison of these systems as well as configuration influence on SO detection is needed to enable robust applications. T...
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ژورنال
عنوان ژورنال: Analusis
سال: 2000
ISSN: 0365-4877,1286-482X
DOI: 10.1051/analusis:2000280475